ELISA试剂盒分析氨肽酶A抗体报告

ELISA试剂盒试 剂 组 成
1、精密度微孔板Microtest Plates 96 wells            
2、酶标偶合液Enzyme Conjugate   12.0ml
3、标准品Standard 5 x 1.0ml                    
4、呈色剂A、Substrate  A  6.0ml
5、呈色剂B、Substrate B  6.0ml                  
6、终止液Stop Solution  6.0ml   
7、浓缩洗涤液（1：20）Rinsing Buffer 60.0ml        
8、5倍样品稀释液  dilution 15.0m
英文名称  Anti-Aminopeptidase A/BP1/CD249 
中文名称  氨肽酶A抗体 
别    名  Aminopeptidase A; AMPE_HUMAN; AP-A; APA; CD249; Differentiation antigen gp160; EAP; EC 3.4.11.7; ENPEP; Glutamyl aminopeptidase; Gp160; Ly51; Ly51/6C3 antigen. 
浓    度  1mg/1ml 
规 格  0.2ml/200μg  
抗体来源  Rabbit  
克隆类型  polyclonal 
交叉反应  Human, Mouse, Rat, Dog, Cow, Sheep
产品类型  一抗    
研究领域  细胞生物 发育生物学 干细胞 淋巴细胞 b-淋巴细胞  
蛋白分子量  predicted molecular weight: 109kDa 
性    状  Lyophilized or Liquid 
免 疫 原  KLH conjugated synthetic peptide derived from human Aminopeptidase A 
亚    型  IgG 
纯化方法  affinity purified by Protein A 
储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 
产品应用   WB=1:100-500  ELISA=1:500-1000  IHC-P=1:100-500  IHC-F=1:100-500  Flow-Cyt=1:100-500  ICC=1:100-500  IF=1:100-500 
（石蜡切片需做抗原修复） 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
ELISA试剂盒氨肽酶A抗体产品介绍 Aminopeptidase A, also designated APA, gp160 human kidney differentiation antigen, glutamyl aminopeptidase, or enpep, is a differentiation-related kidney glycoprotein. As a cell surface, zinc-dependent metalloprotease, Aminopepti-dase A specifically cleaves amino-terminal acidic residues from peptide substrates such as Angiotensin II. APA is expressed on the surface of epithelial cells of the glomerulus and proximal tubule cells of the human nephron, where it may mediate the constitutive trafficking of Glut4-containing vesicles. These Glut4-containing vesicles are tissue-specific secretory-like microsomal structures that mediate insulin-dependent translocation of GLUT4 to the cell surface in fat and muscle cells. Mutations in the gp160/APA gene, including loss of protein expression or enzymatic activity, occur in 20% of primary clear cell renal carcinomas. 
Function : Appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.
Subcellular Location : Membrane.
Tissue Specificity : Expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. Also found in a variety of other tissues.
Similarity : Belongs to the peptidase M1 family.
Database links : UniProtKB/Swiss-Prot: Q07075.3